华南农业大学学报2011，Vol.32Issue(2)：57-61,5.
用启动子融合GUS的方法分析OsUgP1基因的表达模式
Analysis of Expression Profile of OsUgpl Gene by Promoter-GUS Chimeric Expression
摘要
Abstract
Promoter of one UDP-glucose pyrophosphorylase gene (OsUgpl) was cloned,fused with β-glucuronidase(GUS) reporter gene and introduced intro rice. Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice. The result showed that GUS expressed in root, stem,leave, palea, lemma, ovary, stigma, anther at meiosis stage, developing endosperm and embryo. The expression of OsUgpl gene in developing seed was compared with that of OsUgp2 gene. An eminent difference between OsUgpl gene and OsUgp2 gene was found. OsUgpl gene expressed in endosperm and embryo in the seed 5 - 20 d after flowering, but OsUgp2 gene almost did not express. The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions, and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.关键词
尿苷二磷酸葡萄糖焦磷酸化酶/启动子/β-葡萄糖苷酸酶(GUS)Key words
UDP-glucose pyrophosphorylase/ promoter/ β-glucuronidase (GUS)分类
生物科学引用本文复制引用
苏珍霞,欧广亮,李奕恒,穆虹..用启动子融合GUS的方法分析OsUgP1基因的表达模式[J].华南农业大学学报,2011,32(2):57-61,5.基金项目
国家自然科学基金(30370800) （30370800）
