首页|期刊导航|中国神经再生研究（英文版）|Mutated recombinant human glucagon-like peptide-1 induces differentiation of PC12 cells
中国神经再生研究（英文版）2011，Vol.6Issue(6)：457-461,5.DOI:10.3969/j.issn.1673-5374.2011.06.010
Mutated recombinant human glucagon-like peptide-1 induces differentiation of PC12 cells
Mutated recombinant human glucagon-like peptide-1 induces differentiation of PC12 cells
摘要
Abstract
"Glucagon-like peptide-1 (GLP-1) and its long-acting analogues have neuroprotective and neurotrophic properties and are emerging as potential treatments for neurodegenerative diseases. Its short half-life has limited the application of GLP-1 in the clinic. We generated a mutated form of human GLP-1 (mGLP-1) using site-directed mutagenesis and gene recombination techniques, and found that these modifications significantly prolonged the biological half-life of GLP-1 compared with native GLP-1 (nGLP-1). This study investigated the role of mGLP-1 on inducing PC12 cell differentiation. mGLP-1 induced PC12 cell differentiation with neurite outgrowth and increased the expression of growth-associated protein-43 and neuronal class III ?-tubulin, and significantly increased cyclic adenosine monophosphate level. No significant difference was found between mGLP-1 and nGLP-1. The results indicate that mGLP-1 activates the GLP-1 receptor, induces PC12 cell differentiation, and has neurotrophic effects.关键词
glucagon-like peptide-1/mutated glucagon-like peptide-1/differentiation/PC12 cellsKey words
glucagon-like peptide-1/mutated glucagon-like peptide-1/differentiation/PC12 cells引用本文复制引用
Jin Wu,Lan Zhang,Zhongwei Sun,Gang Huang,Jing Huang,Bing Mei..Mutated recombinant human glucagon-like peptide-1 induces differentiation of PC12 cells[J].中国神经再生研究（英文版）,2011,6(6):457-461,5.基金项目
Supported by:the National Natural Science Foundation of China,No.81070876 （）
the Shanghai Science and Technology Commission,No.10JC1411200 （）
the Fundamental Research Funds for the Central Universities （）
