解剖学杂志2011，Vol.34Issue(2)：156-159,175,5.
异种细胞移植对大鼠损伤脊髓降钙素基因相关肽表达的影响
Effects of xenotransplantation of microencapsulated sciatic nerve tissue cells on the expression of calcitonin gene-related peptide after spinal cord injury in rats
摘要
Abstract
Objective: To observe the effects of xenotransplantation of the microencapsulated sciatic nerve tissue/cella on the expression of calcitonin gene-related peptide (CGRP) after spinal cord injury (SCI) in rats.Methods: Bilateral sciatic nerves of rabbits were preconditioned for 1 week were dissected under sterile conditions and made into nerve tissue/cells suspension which was mixed with 1.5％ sodium alginate solution.The mixture was then extruded using a droplet generation device into 20 mmol/L barium chloride solution to form barium-alginate capsules.Microencapsulated nerve tissue/cells suspension was implanted into the injured sites of the spinal cord in rats.By immunohistochernstry, the expressions of CGRP were observed.Results: The number of CGRP-positive cells in the right ventral horn of spinal cord of rats reduced remarkably in each group after SCI, and it gradually recovered in varying degrees 1 week later.The difference of the number of CGRP-positive cells between the microcapsule group and the cell group was not remarkable, but both of them existed in the distinction when compared with the injury group (P ＜0.05).At 2, 4 weeks and 8 weeks after SCI, compared to the cell group and injury group,the number of CGRP-positive cells in the microcapsule group increased obviotrsly.Conclusion: The xenotransplantation of the microencapsulated sciatic nerve tissue/cells promotes the expression of CGRP after SCI in rats, benefiting the regeneration of neuron.关键词
微囊/异种移植/降钙素基因相关肽/施万细胞/脊髓损伤Key words
microcapsule/ xenotransplantation/ calcitonin gene-related peptide/ Schwann cells/ spinal cord injury引用本文复制引用
段平国,孙贵才,刘德明..异种细胞移植对大鼠损伤脊髓降钙素基因相关肽表达的影响[J].解剖学杂志,2011,34(2):156-159,175,5.基金项目
国家自然科学基金(30060034) （30060034）
江西省教育厅科技基金(GJJ10350) （GJJ10350）
江西省卫生厅科技基金(20081098) （20081098）
