解剖学杂志2011，Vol.34Issue(2)：199-202,4.
锐钛矿型纳米二氧化钛颗粒对人肺腺癌A549细胞的毒性效应
Cytotoxicity of anatase TiO2 nanoparticles on A549 celIs
摘要
Abstract
Objective: To investigate the toxic effects of anatase TiO2 nanoparticles in vitro. Methods: A549 cells were incubated together with different concentrations of anatase TiO2 uanoparticles for 4 hours, and then collected. CCK-8 assay was performed to evaluate the viability of TiO2-treated cells after a 4-hour exposure. Laser scanning confocal microscope was used to observe the reactive oxygen species (ROS) production after TiO2 nanoparticles entering A549 cells. The cellular ultrastructure changes caused by TiC2 nsnoparticles were observed under a transmission electron microscope. Results: The viability of A549 cells was reduced after being exposed to TiO2 in a dose-dependent manner. ROS was observed under a confocai microscope, and the levels of ROS had a dose-effect relationship with anatase TiO2 nanoparticles. The distribution of anatase TiO2 nsnoparticles was found in cluster in A549 cells under a transmission electron microscope and caused great damage to cell ultrastructure. Conclusion: Anstase TiO2 nanoparticles can result in cytotoxicity in A549 cells through oxidative stress, and thus cause damage to cell ultrastructure and inhibit cell growth.关键词
锐钛矿纳米二氧化钛颗粒/A549细胞/细胞存活率/活性氧/超微结构Key words
TiO2 nanoparticles/ A549 cells/ cell viability/ reactive oxygen species/ ultrastructure引用本文复制引用
李晓林,汤莹,金婵,范晓燕,许珊,晋若冰,林方兴,杨勇骥..锐钛矿型纳米二氧化钛颗粒对人肺腺癌A549细胞的毒性效应[J].解剖学杂志,2011,34(2):199-202,4.基金项目
国家重点基础研究发展计划(2006CB932505) （2006CB932505）
上海市科学技术委员会纳米专项(0752nm020) （0752nm020）
