中国中医药信息杂志2011，Vol.18Issue(3)：44-46,3.DOI:10.3969/j.issn.1005-5304.2011.03.016
消痰散结方对血管生成拟态相关蛋白表达的影响
Effects of Xiaotan Sanjie Decoction on the Expression of Vasculogenic Mimicry Associated Proteins
周伟 1李勇进 1魏品康 1陈天池 1巨大维1
作者信息
- 1. 中国人民解放军第二军医大学长征医院中医科,上海,200003
- 折叠
摘要
Abstract
Objective To investigate the effects of Xiaotan Sanjie Decoction (XTSJD), a compound traditional Chinese herbal medicine, on the expression of vasculogenic mimicry (VM) associated proteins.Methods The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of human gastric cancer into 30 BALB/c nude mice.After modeling, the tumor-bearing mice were randomly divided into normal saline (NS) group, XTSJD group and doxycycline hyclate (DH) group, 10 mice in each group.And the mice were administered with corresponding medicine by garage for 4 weeks.Then all mice were humanely euthanized with quick cervical dislocation.The morphology of VM was observed.The amount of VM was counted.Expression of vasculogenic mimicry associated proteins MMP-2, MMP-9 and LN-5γ2 were detected by immunohistochemical method.Results Nude mice with transplanted tumors of human gastric cancer can form VM.The amount of VM in XTSJD group and DH group was significantly less than that in NS group (P＜0.01).The expression of MMP-2, MMP-9 and LN-5γ2 in XTSJD group and DH group was significantly lower than that in NS group (P＜0.01).Conclusion XTSJD can inhibit the formation of VM in xenografted tumor of nude mice.The mechanism may be related with down-regulation of MMP-2, MMP-9 and LN-5γ2 expression.关键词
消痰散结方/胃肿瘤/动物模型/血管生成拟态/基质金属蛋白酶分类
医药卫生引用本文复制引用
周伟,李勇进,魏品康,陈天池,巨大维..消痰散结方对血管生成拟态相关蛋白表达的影响[J].中国中医药信息杂志,2011,18(3):44-46,3.
