山西医科大学学报2011，Vol.42Issue(5)：370-374,5.DOI:10.3969/J.ISSN.1007-6611.2011.05.003
兔心肌梗死模型的建立及心功能评价
Establishment of myocardial infarction rabbit model and the evaluation of cardiac function
摘要
Abstract
Objeaive To explore a better method for establishing an economic and efficient model of myocardial infarction in rabbits,and to evaluate its cardiac function after myocardial infarction. Methods After anesthetized with pentobarbital sodium. the left anterior descending branch was ligated in a sterile condition without using tracheal intubation and breathing machine. The changes of ECG were recorded by hiological workstation. The level of creatine kinase-MB( CK-MB )and cardiac troponin T( cTnT ) in blood serum were detected hefore and after ligation for 4 h. TTC and Even ' s blue staining were used to calculate the left ventricle myocardial size.infarction size and area at risk respectively. The postoperative cardiac function was determined and compared with preoperative cardiac function after 4 weeks. Results The mortality of model rabbits was 13. 9％ . After ligation of LAD , ECG showed the ischemia in most rahbits. The level of cre.atine kinase-MB and cardiac troponin T in blood serum raised significantly compared with the preoperative level. The infracted area of myocardium was ( 23. 6 ±3. 5 )％ and the ischemia area was ( 41. 9±7. 2 )％ of left ventricle. The infracted area was ( 56. 2 ±8. 1 )％ of the ischemia area. The statistically significant differences were found in the left ventricular end-systolic diameter,left ventricular end-diastolic diameter.left ventricular ejection of fraction and left ventricular fractional shortening before and after surgery( P ＜0. 05 ). Conclusion It is an efficient and economic method for establishing the model of myocardial infarction by sternotomy with the advantages of a higher survival rate, no injury of membrana pleuralis and avoiding the tracheal intubation and breathing machine. After establishing the model of myocardial infarction. the cardiac function can be estimated by echocardiogram.关键词
疾病模型/心肌梗死/心功能/兔分类
医药卫生引用本文复制引用
杨明,高长青,李力兵,刘多辉..兔心肌梗死模型的建立及心功能评价[J].山西医科大学学报,2011,42(5):370-374,5.基金项目
全军"十一五"科技攻关基金资助项目(06G112) （06G112）
