北京大学学报（医学版）2011，Vol.43Issue(2)：183-188,6.DOI:10.3969/j.issn.1671-167X.2011.02.005
膜联蛋白5对大鼠睾丸间质细胞中类固醇急性调节蛋白和睾酮合成相关酶表达的影响
Effect of annexin 5 on StAR and testosterone synthesis related enzyme in rat Leydig cells
摘要
Abstract
Objective : To investigate the effect of annexin 5 on the expressions at mRNA levels and protein levels of StAR, P450scc, 3β-HSD, 17α-hydroxylase and 17β-HSD in rat Leydig cells. Method: The primary rat Leydig cells were cultured for 24 h and then stimulated with 10 -9 mol/L annexin 5 for 12 h and 24 h respectively. Cellular total RNA and total protein were extracted respectively. The expressions of StAR, P450scc, 3β-HSD , and 17α-hydroxylase and 17β-HSD10 mRNA were detected by reverse transcription-polymerase chain reaction ( RT-PCR) and the protein levels were detected by Western blotting. Results : Compared with the control group, at the mRNA level, after being treated with annexin 5 for 12 h, only 17β-HSD10 expression had a 26％ increase (P ＜0. 05 ) while the others had no significant difference. The expressions of StAR, P450scc and 3β-HSD elevated 55％ , 69％ and 59％ (P ＜0. 05 ) respectively , and 17β-HSD10 increased 104％ ( P ＜ 0. 01 ) while 17α-hydroxylase had no significant difference after being treated with annexin 5 for 24 h. At the protein level, after being treated with annexin 5 for 12 h, 17β-HSD expression had a 39％ increase (P ＜0. 05 ). After 24 h, P450scc, 3β-HSD and 17β-HSD elevated 35％ , 88％ (P ＜0. 05) and 47％ (P ＜0.01) respectively while StAR had no significant difference. Conclusion: Annexin 5 regulates testosterone synthesis by affecting the expressions of P450scc, 3 β-HSD and 17β-HSD at gene and protein levels.关键词
膜联蛋白5/睾丸间质细胞/胆固醇侧链断裂酶/17-羟甾类脱氢酶类分类
医药卫生引用本文复制引用
陶晓倩,姚兵,林钗英,柳海燕,时姗姗,路榕,黄宇烽,李晓军,戈一峰,崔英霞..膜联蛋白5对大鼠睾丸间质细胞中类固醇急性调节蛋白和睾酮合成相关酶表达的影响[J].北京大学学报（医学版）,2011,43(2):183-188,6.基金项目
国家自然科学基金(30770801)、全军"十一五"医药卫生科研基金(06H011)和南京军区创新课题(07Z031)资助项目 （30770801）
