北京大学学报（医学版）2011，Vol.43Issue(2)：199-203,5.DOI:10.3969/j.issn.1671-167X.2011.02.008
荧光原位杂交检测乳腺癌人表皮生长因子受体2基因扩增及与临床病理特征的关系
Detection of human epidermal growth factor receptor 2 gene in invasive breast cancer by fluorescence in situ hybridization and research of its association with tumor characteristics
摘要
Abstract
Objective : To investigate the concordance of detecting human epidermal growth factor receptor 2 ( HER-2 ) status of patients with invasive breast cancer by fluorescence in situ hybridization ( FISH) for gene amplification and immunohistochemistry (IHC) for protein overexpression and to evaluate the association between tumour characteristics and HER-2 gene amplification. Methods : The HER-2 status was evaluated in 41 invasive ductal breast carcinomas by both FISH and IHC. The associations between HER-2 gene amplification and other clinicopathological factors, tumour grade, estrogen receptor ( ER) , tumor emboli, lymph node status, were evaluated using the X2 test. Results: HER-2 amplifications by FISH were seen in 15 patients, including 8 IHC + + + patients (88. 89％ ,8/9 of all + + + cases) , 6 IHC + + patients (42. 68％ ,6/14 of all + + cases) , 1 IHC +patient (8. 33％ ,1/12 of all + cases) and none IHC negative patient (0％ ,0/6 of all negative cases). ER negative or weak positive breast cancers were more often HER-2 FISH positive than ER strong positive cancers ( P = 0. 018) . HER2 amplifications were more often negative in tumor emboli negative or lymph node negative patients than in tumor emboli positive or lymph node positive patients ( P = 0. 000 , P = 0. 025 ) . There was no statistical difference in HER-2 gene amplification among different grades of tumors ( P = 0. 095 ) . Conclusion : HER-2 amplification in breast cancer can be determined by FISH accurately. ER is negatively associated with HER-2 amplification, whereas tumor emboli and lymph node status are positively associated with HER-2 .关键词
乳腺肿瘤/原位杂交,荧光/受体,表皮生长因子分类
医药卫生引用本文复制引用
苏静,杨郁,马晓龙,由江峰,杜娟,柳剑英,郑杰..荧光原位杂交检测乳腺癌人表皮生长因子受体2基因扩增及与临床病理特征的关系[J].北京大学学报（医学版）,2011,43(2):199-203,5.基金项目
卫生部行业基金(200802011)和北京大学医学部新教师科研启动基金(BMU20090527-123)资助 （200802011）
