北京大学学报（医学版）2011，Vol.43Issue(2)：234-237,4.DOI:10.3969/j.issn.1671-167X.2011.02.014
异丙酚对大鼠海马神经元低电压激活钙电流的抑制作用
Suppressive effect of propofol on low-voltage-activated calcium currents in rat hippocampal neurons
摘要
Abstract
Objective : To investigate the effect of propofol on the low-voltage-activated calcium currents [ⅠCa(LVA) ] in rat hippocampal neurons. Methods: Hippocampal neurons were prepared from Wistar rats and cultured. ⅡCa(LVA) was recorded using whole-cell patch clamp technique. Different concentrations of propofol were added to the culture. The effect of propofol on ⅠCa( LUA) was evaluated. Results : ⅠCa( LVA) was inhibited by propofol in a concentration-dependent manner. Propofol 3 μmol /L had no effect on ⅠCa( LVA)· Propofol 10, 30, 100 and 300 μmol/L reduced peak ⅠCa(LVA) by ( 12. 6 ±4. 1 )％ , ( 29. 2 ±5. 7)％ , (36. 6 ±5. 3 ) ％ , ( 31. 6 ±2. 6) ％ respectively with a mean IC50of 16. 8 μmol/L and Hill coefficient of 0. 15. The V1/2 of activation curve was shifted from ( - 10 ±1 )mV to ( -11 ±2 ) mV. K was 12 ±1 and 8 ±1. The V1/2 0f inactivation curve was shifted from ( - 25 ± l) mV to ( - 25 ±5) mV. K was 15 ± 1 and 16 ±3. Conclusion : Propofol produces significant inhibition of ⅠCa (LVA) calcium currents in the central neurons which may partly explain the anesthefic action of propofol on thecentral nervous system.关键词
异丙酚/海马/神经元/膜片钳术/钙通道分类
医药卫生引用本文复制引用
谭宏宇,叶铁虎,马士平,王晓良..异丙酚对大鼠海马神经元低电压激活钙电流的抑制作用 [J].北京大学学报（医学版）,2011,43(2):234-237,4.基金项目
国家自然科学基金(30672030)资助 （30672030）
