医药导报2011，Vol.30Issue(6)：699-701,3.DOI:10.3870/yydb.2011.06.002
盐酸小檗碱与8-羟基二氢小檗碱对大鼠脑组织与血清中乙酰胆碱酯酶活性的影响
Effects of Berberine Hydrochloride and 8-hydroxy Dihydroberberine on the Activity of Acetylcholinesterase (AChE) in the Cerebral Tissue and Serum of Rats
摘要
Abstract
Objective To investigate the inhibitory effects of monomer berberine hydrochloride and its structuremodified compound 8-hydroxy dihydroberberine on the activity of acetylcholinesterase ( AChE ) in the cerebral tissue and serum of rats.Methods The rats were sacrificed by cervical dislocation after the blood was collected from abdominal aorta.The cerebral tissue was homogenized and the total protein level in it was measured using biuret.The AChE activity in cerebral tissue and serum was detected by enzymatic method.Results The inhibitory rate of AChE activity in cerebral tissue by berberine hydrochloride was 52.57％ -82.22％ whereas 32.78％ -96.14％ by 8-hydroxy dihydroberberine.The inhibitory rate of serum AChE activity by berberine hydrochloride was 8.44％ -99.59％ and 11.72％ -99.59％ by 8-hydroxy dihydroberberine.The inhibitory extent was in a dose-effect relationship with berberine.Conclusion Both berberine hydrochloride and 8-hydroxy dihydroberberine could inhibit AChE activity in cerebral tissue and serum obviously, which may be a new target for the treatment of diabetes.关键词
小檗碱,盐酸/8-羟基二氢小檗碱/乙酰胆碱酯酶/脑组织/血清/大鼠分类
医药卫生引用本文复制引用
王开富,徐丽君,邹欣,陆付耳..盐酸小檗碱与8-羟基二氢小檗碱对大鼠脑组织与血清中乙酰胆碱酯酶活性的影响[J].医药导报,2011,30(6):699-701,3.基金项目
国家新药创制重大专项基金资助课题(基金编号:2009zx09301-014) （基金编号:2009zx09301-014）
