山西医科大学学报2011，Vol.42Issue(5)：406-408,3.DOI:10.3969/J.ISSN.1007-6611.2011.05.012
时间分辨荧光免疫分析法测定乙肝HBsAg的临床意义
Clinical significance of time-resolved fluoroimmunoassay for detecting hepatitis B virus surface antigen
蔡军 1刘晓丽1
作者信息
- 1. 河南省人民医院检验科,郑州,450003
- 折叠
摘要
Abstract
Objective To evaluate the clinical significance of time-resolved fluoroimmunoassay ( TRFIA )in detection of hepatitis B virus surface antigen.Methods A total of 206 serum samples were detected by TRFIA and ELISA methods respectively.And each 20 samples of high,medium and low HBsAg concentration were re-determined by the two methods.Results ①Of 206 samples,98 samples were positive for HBsAg with TRFIA assay, and 85 samples were positive for HBsAg with ELISA assay.The detection rate was statistically different between two methods(x2 = 11.08,P ＜ 0.01 ).Concordance rate between TRFIA and ELISA test was 93.69％.②In each 2 0 samples of high and medium concentrations of HBsAg , all were positive by TRFIA and ELISA , and there was no significant difference between two methods( P ＞ 0.05 ).However, the detection rate with TRFIA assay was higher than that with ELISA assay in 20 samples of low concentration ( x2 = 4.17,P ＜ 0.05 ).Conclusion TRFIA assay is more sensitive and has wider linear range in quantitative detection of hepatitis B virus.Moreover,TRFIA assay has more advantages than ELISA in detection of low concentration samples.关键词
时间分辨荧光免疫分析技术/酶联免疫吸附试验/乙肝表面抗原分类
医药卫生引用本文复制引用
蔡军,刘晓丽..时间分辨荧光免疫分析法测定乙肝HBsAg的临床意义[J].山西医科大学学报,2011,42(5):406-408,3.
