中国药理学通报2011，Vol.27Issue(6)：760-763,4.DOI:10.3969/j.issn.1001-1978.2011.06.006
橘红素增强5-氟尿嘧啶抑制人胃癌AGS细胞增殖的作用及其机制研究
Tangeretin enhances the proliferation inhibition of 5-fuorouracil in human gastric cancer AGS cells
摘要
Abstract
Aim To study the effects and mechanismsof tangeretin combined with 5-fuorouracil ( 5-Fu ) onproliferation of human AGS gastic cancer cells. Meth-ods MTT assay was used to detect the growth inhibi-tory rates of human AGS gastric cancer cells treatedwith 5-Fu and tangeretin combined with 5-Fu. The cellcycle distribution was measured by flow cytometry. Theprotein expressions of p21 and p53 were detected byWestern blot. Results MTT assay showed 5-Fu inhib-ited the growth of AGS gastric cancer cells in a dose-dependent manner ( P ＜ 0. 05 , P ＜ 0. 01 ) and inhibi-tory rates of tangeretin combined with 5-Fu was higherthan those of 5-Fu alone ( P ＜ 0. 01 ). Tangeretin com-bined with 5-Fu arrested AGS cells in S phase com-pared to control group ( P ＜ 0. 01 ). Western blotshowed that tangeretin combined with 5-Fu increasedexpressions of p53 and p21 protein compared to thattreated with 5-Fu alone ( P ＜ 0. 01 ). ConclusionsTangeretin combined with 5-Fu can enhance the prolif-eration inhibition of 5-Fu in human gastric cancer AGScells. S phase arrest and upregulation of p53 and p21protein might be the mechnisms.关键词
橘红素/5-氟尿嘧啶/胃癌/增殖/细胞周期/p53/p21分类
医药卫生引用本文复制引用
鲁岚,董杨,施建蓉,吴大正..橘红素增强5-氟尿嘧啶抑制人胃癌AGS细胞增殖的作用及其机制研究[J].中国药理学通报,2011,27(6):760-763,4.基金项目
国家科技重大专项资助项目(No 2009ZX09311-003) （No 2009ZX09311-003）
上海市教委高校高水平项目建设资助项目(No 2008GSP19) （No 2008GSP19）
上海市教委重点学科资助项目(No J50305) （No J50305）
上海市教委预算内科研资助项目(No 09JW21) （No 09JW21）
