中国药理学通报2011，Vol.27Issue(6)：764-769,6.DOI:10.3969/j.issn.1001-1978.2011.06.007
吲哚-3-原醇对复合因素所致肝纤维化大鼠的治疗作用及部分机制研究
Therapeutic effects of indole-3-carbinol on multiple hepatotoxic factors-induced liver fibrosis in rats
摘要
Abstract
Aim To investigate the effects of indole-3carbinol ( I3C ) on multiple hepatotoxic factors-induced liver fibrosis in rats and its possible mechanism. Methods Male Wistar rats were divided randomly into six groups , a normal control group , a model control group ,a I3C control group and fibrotic groups treated with three different doses of I3C. The fibrotic model was induced by multiple hepatotoxic factors, including carbon tetrachloride. ethanol and high fat/low proteins for 4 weeks . After completing models , rats in treatment groups were administered I3C 3. 6 and 12 mg · kg-1 through intraperitoneal injection, daily for 10 days.Hepatic activity of superoxide dismutase ( SOD ), hepatic malondialdehyde ( MDA ) and hepatic hydroxy proline ( Hyp ) content were determined. The liver histology and immunohistochemistry with a-smooth muscle actin ( α-SMA ) were assayed. Meanwhile. the expression of matrix metalloproteinases-2 ( MMP-2 ) in liver was analyzed by Western blot. Results Compared with the fibrosis control group, hepatic content of Hyp was decreased by 13 C treatment , and the activity of hepatic SOD was increased , especially in groups with I3C at the dose of 12 mg · kg-1( P ＜0. 01 ). In addition,histopathological changes, especially changes of myofibroblast proliferation, were reduced remarkably( P ＜ 0. 05 ) and the expression of a-SMA was downregulated in the I3C-treated groups. As compared with fibrosis control group, the expression of MMP-2 was increased by 13C treatment. Conclusion I3C can promote the recovery of liver fibrosis in vivo. The mechanism might be due to protective effect for hepatocytes, reduction of the number of activated HSC and acceleration of collagen degradation.关键词
吲哚-3-原醇/肝纤维化/肝星状细胞/胶原/活化/氧化应激分类
医药卫生引用本文复制引用
高爱梅,平洁,汪晖..吲哚-3-原醇对复合因素所致肝纤维化大鼠的治疗作用及部分机制研究[J].中国药理学通报,2011,27(6):764-769,6.基金项目
国家自然科学基金资助项目(No 30371666,81001466),湖北省自然科学基金资助项目(No 2008CDB117) （No 30371666,81001466）
