中国药理学通报2011，Vol.27Issue(6)：769-773,5.DOI:10.3969/j.issn.1001-1978.2011.06.008
栀子苷对缺氧/复氧小胶质细胞TLR4通路的影响
Geniposide on TLR4 pathway in cultured rat microglia with hypoxia/reoxygenation
摘要
Abstract
Aim To study the effect of hypoxia / reoxygenation on TLR4 , MyD88, NF-KBp65, p-ERK1/2,p-IκBα and the role of geniposide in the primary cultured microglia. To explore the molecular mechanism of the treatment of cerebral ischemia. Methods The hypoxia/reoxygenation model was established and treated with geniposide of 125, 250 and 500 μmol · L-1,the expression of TLR4 mRNA with the reverse transcription polymerase chain reaction was detected, and the protein of TLR4, p-IκB, p38, p-ERKI/2 with Western blot, MyD88 and NF-κBp65 were observed with immunofluorescence double staining. Results The hypoxia/reoxygenation activates the TLR4 pathway and the protein of TLR4, MyD88. NF-κBp65, pERK1/2, p38 and p-IKB through the MyD88-dependent signaling, the group geniposide of 250 and 500 μmol · L-1 inhibited the activation. Conclusions The hypoxia/reoxygenation activates the protein of TLR4 pathway. Geniposide plays anti-inflammatory role through inhibition of protein on the TLR4 pathway, and promotes the recovery of cerebral congestion.关键词
缺氧/复氧/小胶质细胞/TLR4通路/栀子苷/MyD88/NF-κBp65/p-ERK1/2/p-IκBα/p38分类
医药卫生引用本文复制引用
侯金才,张鹏,刘建勋,韩笑,李丹,王秀辉..栀子苷对缺氧/复氧小胶质细胞TLR4通路的影响[J].中国药理学通报,2011,27(6):769-773,5.基金项目
国家自然科学基金资助项目(No 30772755,81073060) （No 30772755,81073060）
国家科技重大专项课题(No 2009ZX09103-317) （No 2009ZX09103-317）
博士后科学基金资助项目(No 20100470514) （No 20100470514）
