中国药理学通报2011，Vol.27Issue(6)：806-809,4.DOI:10.3969/j.issn.1001-1978.2011.06.016
左旋精氨酸通过上调Th1应答介导抗疟保护性免疫
L-Arg mediated anti-malaria protective immunity by enhancing Th1 immune responses
摘要
Abstract
Aim Investigate the effects of L-Arg during blood-stage infection by P. y17XL in BALB/c mice.Methods Mice were orally administrated with /-Arg ( 1. 5 g · kg-1 ) 7 days before P. y17XL infection ( LArg group ). The control group received the same volume normal saline at the same time points before P.y17XL infection. Parasitemia were monitored by Giemsa stained thin - smear microscopy . Flow cvtometry was introduced to detect the subsets of splenic CD4+ CD69+T. F4/80+ CD36+ macrophages on day 3, 5 post infection ( pi ). The level of IFN-γ and NO in the supernatant of splenocytes culture was detected by ELISA and Griess reaction, respectively. Results Pre-treatment of mice with L-Arg significantly decreased the parasitemia and elongated their survival time after infection. The number of F4/80+ CD36+ macrophages and CD4+ CD69+ T cells was obviously higher in L-Arg group. Enhanced IFN-γ and NO production induced by L-Arg in spleen cells of infected mice revealed that the Thl immune response was stimulated against malaria infection. Conclusion L-Arg can induce protective Th1 immune responses to control the proliferation of malaria parasites during the bloodstage of P. y17XL infection.关键词
疟疾/精氨酸/致死型约氏疟原虫/免疫调节/Th1免疫应答/BALB/c小鼠分类
医药卫生引用本文复制引用
潘艳艳,刘军,李莹,延娟,冯永辉,王各各,冯辉,郑丽,曹雅明..左旋精氨酸通过上调Th1应答介导抗疟保护性免疫[J].中国药理学通报,2011,27(6):806-809,4.基金项目
国家自然科学基金资助项目(No 30800962) （No 30800962）
