中国药理学通报2011，Vol.27Issue(6)：809-814,6.DOI:10.3969/j.issn.1001-1978.2011.06.017
CCl4诱导的大鼠肝纤维化模型肝纤维化逆转与MAPK信号通路的研究
Dynamic expression of MAPK signaling pathway on recovery hepatic fibrosis rats induced by CCl4
摘要
Abstract
Aim To investigate the expression of MAPK signaling pathway in CCl4-induced recovery hepatic fibrosis rats and hence to explore their regulatory role. Methods SD rats were injected with CCl4 for 12 weeks to reproduce spontaneous reversal of liver fibrosis animal models. These models were divided into normal group, model group, 2-week recovery , 4-week recovery, 6-week recovery and 8-week recovery according to the experimental design. ALT, AST, Hyp, LN,PⅢ NP and Masson stainning of liver pathology of each group were verified. Western blot was used to detect the protein expression. Results The results of ALT,AST, Hyp, LN, P Ⅲ NP and Masson stained tissue sections of liver pathology of each group suggested that animal model had been established. The expression of p-ERK1/2 was the lowest in the model group, with the normal group there were significant differences( P ＜0. 01 ). 4-week recovery was highest and significantly difference compared with the model group( P ＜ 0. 01 ) .then gradually reduced to normal level. p-JNK1/2 was the lowest in the model group( P ＜ 0. 01 ). then increased in recovery groups( P ＜0. 05 ). The expression of p-p38 was the highest in the model group( P ＜0. 01 ), and gradually decreased in recovery groups( P ＜ 0. 05 ). The coefficient correlation of p-ERK/ERK and Hyp r = -0. 46 . P = 0. 003 , the coefficient correlation of p-JNK/JNK and Hyp r = - 0. 53 ,P = 0. 0004 .and the coefficient correlation of p-p38/p38 and Hyp r =0. 81 ,P = 0. 0005. Conclusion The MAPK signaling pathway may play a pivotal role in the spontaneous reversibility of hepatic fibrosis.关键词
肝纤维化/有丝分裂原活化蛋白激酶/细胞外信号调节激酶/c-Jun氨基末端激酶/p38丝裂原活化蛋白激酶/信号通路分类
医药卫生引用本文复制引用
李政通,李俊,黄成,李浩,章圣鹏,黄艳,陶辉..CCl4诱导的大鼠肝纤维化模型肝纤维化逆转与MAPK信号通路的研究[J].中国药理学通报,2011,27(6):809-814,6.基金项目
国家自然科学基金资助项目(No 81072686,30873081) （No 81072686,30873081）
高等学校博士学科点新教师类专项科研基金资助项目(No 20103420120001) （No 20103420120001）
