中国药理学通报2011，Vol.27Issue(6)：831-834,4.DOI:10.3969/j.issn.1001-1978.2011.06.022
人类胚胎干细胞分化研究
Differentiation of human embryonic stem cell
摘要
Abstract
Aim To study the effect of neural induction medium and RA on human embryonic stem cell differentiation. Methods H9 cells maintained on Matrigel in mTeSRTMI medium were converted to neural cells with chemically defined neural induction medium to test the expression of neural marker proteins , and were treated with retinoid acid ( RA ) to test expression of BIP, an endoplasmic reticulum stress related protein.Results Neural cells were generated from H9 cells successfully with neural induction medium, and expression of BIP was upregulated during the H9 cell differentiation induced by RA. Conclusions Appropriate HESC differentiation provides the potential cell source for generating neural cells and a platform for developmental study.关键词
人类胚胎干细胞/H9细胞/神经分化/视黄酸/BIP/发育分类
医药卫生引用本文复制引用
刘超,刘丽华,方圣云..人类胚胎干细胞分化研究[J].中国药理学通报,2011,27(6):831-834,4.基金项目
美国马里兰州干细胞研究基金(No 2007-MSCRFE-0139-01) （No 2007-MSCRFE-0139-01）
