中国药理学通报2011，Vol.27Issue(6)：855-859,5.DOI:10.3969/j.issn.1001-1978.2011.06.027
PI3K-Akt-mTOR信号通路对Foxp3基因表达的影响
Effect of PI3K-Akt-mTOR signal pathway on the expression of Foxp3
葛圣林 1彭磊磊 1张成鑫1
作者信息
- 1. 安徽医科大学第一附属医院心脏血管外科,安徽,合肥,230022
- 折叠
摘要
Abstract
Aim To investigate the effect of PI3K-AktmTOR signal pathway on the expression of Foxp3 in vivo. Methods 60 Kunming-strain mice at the age of 8 weeks were randomly divided into a control group ( A ) and three experimental groups( B, C , D ). The mice in the groups B,C and D were fed with rapamycin( RAPA ) 0. 2 mg · d-1 ,0. 4 mg · d-1 and 0. 6 mg · d-1 via intragastric administration. The mice in the group A were given with sterile water as the control group. After three weeks, the peripheral blood ( with EDTA anticoagulant ) was collected under sterile conditions and the splenocytes were seperated to generate a single-cell suspension. The levels of CD4 + CD25+ Treg cells in the peripheral blood and splenocytes of mice were detected by flow cytometer. ( The ratio of CD4+ CD25+ Treg cells to CD4+ CD25+ Treg cells ). The expression of Foxp3 mRNA in spleen cells of mice were detected with RT-PCR assay. Variation of p-mTOR expression in spleens were observed with immunohistochemical staining ( SP method ) and the average opticals of protein p-mTOR's expression between experimental groups and control group were tested with computer image analytical technique. Results The levels of Foxp3 mRNA in spleen cells of mice among experimental groups( B, C,D) were 0. 4853 ± 0. 0574, 0. 7886 ± 0. 1085 and 0. 9639 ± 0. 2015 respectively , which were significantly higher than control group A( 0. 1345 ± 0. 0271 )( P ＜0. 05 ); The average opticals of protein p-mTOR's expression among experimental groups ( B, C, D ) were 0. 2326 ± 0. 0431. 0. 1156 ± 0. 0223 and 0. 0556 ± 0. 0041 respectively, which were lower than control group A( 0. 4223 ±0. 0534 )( P ＜ 0. 05 ). Itshowed that there was a negative correlation between the expression of Foxp3 mRNA and protein p-mTOR which were handled through pearson correlation analysis.Conclusion RAPA can enhance the expression of Foxp3 by inhibiting PI3K-Akt-mTOR signal pathway.There is a negative correlation between the activation of PI3K-Akt-mTOR signal pathway and the expression of Foxp3.关键词
雷帕霉素/PI3K-Akt-mTOR/信号通路/Foxp3/CD4+CD25+Treg细胞/p-mTOR分类
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葛圣林,彭磊磊,张成鑫..PI3K-Akt-mTOR信号通路对Foxp3基因表达的影响[J].中国药理学通报,2011,27(6):855-859,5.
