实用医学杂志2011，Vol.27Issue(9)：1526-1528,3.DOI:10.3969/j.issn.1006-5725.2011.09.004
儿茶素对脑胶质瘤细胞U251增殖的抑制作用及机制初探
Inhibitory effect of catechins on proliferation of human glioma U251 cells
梁丽琴 1金刚 2党建章 2代建国 2陈卫平 1张燕2
作者信息
- 1. 330045,南昌市,江西农业大学食品科学与工程学院
- 2. 518055,深圳职业技术学院应用化学与生物技术学院
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect of catechins on proliferation of human glioma U251 cells in vitro and illustrate the potential mechanism. Methods MTT assay was performed to determine the semi-lethal concentration of catechins for U251 cells. Trypan blue staining and Hoechst33442 fluorescent staining were conducted to detect U251 cell apoptosis. U251 cell morphology was ohserved under inverted microscope.Cell cycle status of catechins treated U251 cells was determined by the flow cytometry. Results The semi-lethal concentration of catechins for U251 cells was 62.25μg/mL. Apoptotic bodies appeared in catechins treated U251 cells, and U251 cell cycle was arrested at S stage. The apoptotic U251 cells increased obviously in a dosedependent and time-dependent manner after catechins treatment. Conclusion Catechins can efficiently inhibit U251 cell proliferation, and it is a promising drug candidate for glioma.关键词
脑肿瘤/神经胶质瘤/儿茶素/U251细胞/细胞凋亡引用本文复制引用
梁丽琴,金刚,党建章,代建国,陈卫平,张燕..儿茶素对脑胶质瘤细胞U251增殖的抑制作用及机制初探[J].实用医学杂志,2011,27(9):1526-1528,3.
