南京农业大学学报2011,Vol.34Issue(2):1-7,7.
杂交粳稻亲本穗长和枝梗数性状优异配合力的标记基因型筛选
Screening marker genotypes with elite combining ability for panicle length and branch numbers in parents of hybrid japonica rice( Oryza sativa L. )
摘要
Abstract
In order to improve combining ability of restorer lines more efficiently,SSR marker genotypes with elite combining ability for three panicle traits were screened in this study, by analyzing the data of combining ability and SSR markers in six CMS ( cytoplasmic male sterility)lines and twelve restorer lines that were genotyped using 115 pairs of SSR primers. The combining ability of the 18 parental lines was analyzed for panicle length(PL) ,primary branch number per panicle(PBN) ,second branch number per panicle (SBN) ,using the data of 72 F1 made with North Carolina Ⅱ genetic mating design. Results showed that twenty-three SSR marker genotypes were significantly associated with combining ability of the three traits. Out of the twenty-three, fifteen were associated with one trait, seven with two traits, and one with three traits. Marker genotype RM8254-120/180 showed the positive effects of combining ability in all the three traits, increasing 21.63% of PBN,21. 12% of SBN and 16. 12% of PL in F1,respectively. Among the seven marker genotypes associated with two traits simultaneously, six showed the positive effects of combining ability for both of the two traits, and the other one negative.关键词
粳稻/配合力/SSR标记/穗长/每穗一次枝梗数/每穗二次枝梗数/标记基因型Key words
japonica rice/ combining ability/ SSR marker/ panicle length/ primary branch number per panicle/ second branch number per panicle/ marker genotype分类
农业科技引用本文复制引用
黄殿成,阮方松,刘健,谢辉,赵凯铭,梁奎,江建华,蔡勇林,洪德林..杂交粳稻亲本穗长和枝梗数性状优异配合力的标记基因型筛选[J].南京农业大学学报,2011,34(2):1-7,7.基金项目
国家863计划项目(2010AA101301) (2010AA101301)
国家948项目(2006-G8(4)-31-1) (2006-G8(4)
教育部科技基础条件平台重点资助项目(505005) (505005)