南京农业大学学报2011，Vol.34Issue(2)：18-22,5.
激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)定量分析小麦籽粒锌元素的空间分布
Quantification and spatial distribution of zinc in wheat grains by laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS)
摘要
Abstract
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)is a recently developed technology for examining mineral elements distribution in plant tissue. In this paper, the spatial distribution of zinc in wheat grains was quantified by LA-ICP-MS. Results indicated large spatial variation of zinc concentration in wheat grains. Zinc concentration in seed coat, aleurone layer and ambryo is 192,432 and 292 mg-kg-1, respectively, only 14 mg·kg-1 in endosperm. As for zinc accumulation, zinc amount in seed coat,aleurone layer,ambryo and endosperm account for 24％ ,47％, 11％ and 18％ of total zinc in grain, which implied that zinc content in grains decreased sharply after seed processing (polished). By comparing the measured zinc value of two different methods (i. e. , LA-1CP-MS and ICP-MS after digestion), we also found that the variation of measured zinc concentration between two methods as well as among replications were small, which suggested that LA-ICP-MS is a reliable method for quantifying the spatial distribution of elements in grains.关键词
激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)/小麦籽粒/锌/定量Key words
laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) /wheat grain/zinc/quantification分类
化学化工引用本文复制引用
王云霞,杨连新,W.J.Horst..激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)定量分析小麦籽粒锌元素的空间分布[J].南京农业大学学报,2011,34(2):18-22,5.基金项目
德国研究基金项目(DFG,HO931/23-1) （DFG,HO931/23-1）
国家自然科学基金项目(30671226) （30671226）
江苏政府留学奖学金项目(2009) （2009）
扬州大学科技创新培育基金项目(2009CXJ021) （2009CXJ021）
