昆明医学院学报2011，Vol.32Issue(2)：3-6,4.
体外培养神经干细胞NGF、BDNF和NT-3及其受体trkA、trkB和trkC的表达
Expression of NGF, BDNF, NT-3 and Their Receptors trkA, trkB, trkC in Cultured Neural Stem Cells
摘要
Abstract
Objective To investigate the expression of NGF, BDNF, NT-3 and their receptors trkA,trkB, trkC in cultured Neural stem cells (NSCs). Methods NSCs were isolated from embryonic hippocampus of mice. They were then cultured in vitro and identified by immunohistochemistry using nestin antibody. The expression of mRNA and protein for NGF, BDNF, NT-3 was detected by RT-PCR and immunohistochemistry.Results Significant levels of mRNA were detected for all three factors and their receptor trkA, trkB, trkC by RT-PCR. The proteins of three factors and their receptors were located in cytoplasma found by immunohistochemistry. Conclusion Cultured NSCs express NGF, BDNF, NT-3, and their receptors,suggesting that the three factors play an important role in NSCs development and function maintainance.关键词
小鼠/神经干细胞/神经营养因子/基因表达Key words
Mice, Neural stemcells, Neurotrphic factors/ Gene expression分类
基础医学引用本文复制引用
李玉,王波,林娜,王廷华,习杨彦彬..体外培养神经干细胞NGF、BDNF和NT-3及其受体trkA、trkB和trkC的表达[J].昆明医学院学报,2011,32(2):3-6,4.基金项目
国家自然科学基金资助项目(30260125) （30260125）
