南京农业大学学报2011，Vol.34Issue(2)：38-46,9.
不同系统梨种质遗传多样性与分类关系的SSR分析
Genetic diversity and polygentic relationship among pears reveled by SSR analysis
摘要
Abstract
Genetic diversity and polygentic relationship of 150 cultivars of pear, which belong to Pyrus bretschneideri Rehd. , Pyrus pyrifolia( Burm. f. )Nakai ,Pyrus ussuriensis Maxim. ,Pyrus communis L. ,Pyrus sinkiangensis Yü,wild species and inter-specific hybridization cultivars, were analyzed by SSR markers in this paper. 25 primer-pairs produced a total of 407 alleles, of which 390 were polymorphic with a polymorphism percentage of 95.82％. Prime conbination were 5-25, Heterozygosity ratio was 0.4-0.7, and the similarity coefficient between cultivars ranged from 0. 67 to 1.00. The dendrogram generated from all the 150 accessions by unweighted pair-group method of arithmetic analysis(UPGMA) cluster analysis clearly distinguished Occidental pears from accessions of East Asia. Cultivars of P. communis and inter-specific hybridization with P. communis are clustered into the Occidental clade. The clade of oriental pear formed two main groups, seven subgroups, at the coefficient of 0.708. P. bretschneideri, P. ussuriensis and P.pyrifolia originating in China interveined each other,and did not group independently. Chinese P. pyrifolia showed the most abundant genetic diversity, followed by P. bretschneideri. The cultivars from Japan and the Chinese P. pyrifolia demonstrated a high degree of genetic relationship, and there is no separate group. The genetic relationship of most new cultivar selections show increasingly female parent type or increasingly male parent poly-poly-type.关键词
梨/遗传多样性/亲缘关系/SSRKey words
Pyrus/ genetic diversity/ polygenetic relationship/ SSR分类
农业科技引用本文复制引用
路娟,吴俊,张绍铃,吴华清,张妤艳..不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011,34(2):38-46,9.基金项目
国家公益性行业(农业)科研专项经费(200903044) （农业）
国家自然科学基金项目(30900974) （30900974）
国家梨产业技术体系建设专项经费(CARS-29) （CARS-29）
