分析化学2011，Vol.39Issue(2)：198-202,5.DOI:10.3724/SP.J.1096.2011.00198
光谱法研究鸡蛋溶菌酶热变性与免疫原性的关系
Investigation for Relationship Between Heat Treatment Process and lmmunogenicity of Lysozyme in Egg By Spectroscopic Method
摘要
Abstract
Thermal denaturation for lysozyme protein of egg has been investigated by using circular dichroism spectra and fluorescence spectra systematically. The corresponding immunogenicity of lysozyme protein has been tested with ELISA experiments. In addition, bioinformatics methods have also been used to investigate the spectral characteristics, corresponding immunogenicity change and conformational changes of the protein during thermal denaturation. Results from this study should be useful to the establishment of a spectral method examining the extent of thermal denaturation of the protein.关键词
鸡蛋溶菌酶/免疫原性/热变性/光谱学/生物信息学Key words
Lysozyme of hen's egg/ Immunogenicity/ Heat treatment/ Spectroscopy/ Bioinformatics引用本文复制引用
吴序栎,朱倩倩,成小娟,吴晖,徐宏,刘志刚..光谱法研究鸡蛋溶菌酶热变性与免疫原性的关系[J].分析化学,2011,39(2):198-202,5.基金项目
本文系国家自然科学基金(Nos.30871752,30570421)资助项目 （Nos.30871752,30570421）
