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嫁接对盐胁迫下西瓜幼苗生长和可溶性蛋白表达的影响

阳燕娟 郭世荣 李晶 杜长霞 陈丽芳 王丽萍

南京农业大学学报2011,Vol.34Issue(2):54-60,7.
南京农业大学学报2011,Vol.34Issue(2):54-60,7.

嫁接对盐胁迫下西瓜幼苗生长和可溶性蛋白表达的影响

Effects of grafting on the soluble protein expression in watermelon seedlings under salt stress

阳燕娟 1郭世荣 1李晶 1杜长霞 1陈丽芳 1王丽萍1

作者信息

  • 1. 南京农业大学园艺学院/农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095
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摘要

Abstract

The effects of grafting on the soluble protein content and expression was conducted with a salt-tolerant Bottle Gourd Peel as the rootstock and a salt-sensitive mini-watermelon as the scion.Results shorwed that grafting significantly increased the growth and the content and expression level of soluble protein in leaves under 50 mmol· L-1 NaCl stress,while decreased in the own-root seedlings.Two proteins of 247.7× 103 and 107.9× 103 were expressed only in grafted seedlings, and twelve proteins changed obviously at least,relative molecular weight ( × 103 ) were 244.7,240.0,74.2,61.2,58.1,45.0,42.0, 40.1,36.4,29.1,15.7 and 15.5.Therefore, it was possible that these proteins have relations with grafting improving the salt tolerance in watermelon seedlings.

关键词

嫁接/盐胁迫/小型西瓜/可溶性蛋白/SDS-PAGE

Key words

grafting/ NaCl stress/ mini-watermelon/ soluble protein/ SDS-PAGE

分类

农业科技

引用本文复制引用

阳燕娟,郭世荣,李晶,杜长霞,陈丽芳,王丽萍..嫁接对盐胁迫下西瓜幼苗生长和可溶性蛋白表达的影响[J].南京农业大学学报,2011,34(2):54-60,7.

基金项目

国家重点基础研究发展计划项目(2009CB119001-02) (2009CB119001-02)

国家自然科学基金项目(30900995) (30900995)

江苏省农业三项工程项目(SX(2008)026) (SX(2008)

国家科技支撑计划项目(2008BADA6B07) (2008BADA6B07)

南京农业大学学报

OA北大核心CSCDCSTPCD

1000-2030

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