南京农业大学学报2011，Vol.34Issue(2)：85-90,6.
中国番茄黄化曲叶病毒编码的RNA沉默抑制子AC4蛋白的核酸结合特性
Nucleic acid binding property of RNA silencing suppressor AC4 protein encoded by Tomato yellow leaf curl China virus
摘要
Abstract
The result of Agrobacterium-mediated co-infiltration assay indicated that AC4 protein encoded by Tomato yellow leaf curl China virus (TYLCCNV)isolate Y10 was a RNA silencing suppressor. In order to analyse the mechanism of RNA silencing suppressor AC4, the AC4 gene of TYICCNV-Y10 was obtained by PCR and was linked to multiple cloning sites of a prokaryotic expression vector pET-32a to construct the recombinant vector,pET32a-Y10AC4. The recombinant vector was transformed into Escherichia coli BI21 ( DE3 ) pLysS and TYLCCNV-Y10 AC4 was expressed and purified under natural conditions. The binding property of the expression protein Y10 AC4 with ss/ds-siRNA and long ss/dsRNA were analyzed by electrophoretic mobility shift assay ( EMSA). The results indicated that the fusion protein Y10 AC4 can bind ss-siRNA and long ssRNA,but not ds-siRNA and long dsRNA.关键词
中国番茄黄化曲叶病毒/ACA蛋白/原核表达/电泳迁移率变动试验Key words
Tomato yellow leaf curl China virus(TYLCCNV)/AC4 protein/prokaryotic expression/electrophoretic mobility shift assay分类
生物科学引用本文复制引用
张馨月,章颉,邓凤林,吴建祥..中国番茄黄化曲叶病毒编码的RNA沉默抑制子AC4蛋白的核酸结合特性[J].南京农业大学学报,2011,34(2):85-90,6.基金项目
国家自然科学基金项目(30670087) （30670087）
国家自然科学基金重点项目(30530520) （30530520）
