南京农业大学学报2011，Vol.34Issue(2)：101-106,6.
脱氧雪腐镰刀菌烯醇对BHK-21细胞的线粒体膜电位及Bax和Bcl-2蛋白表达的影响
Effect of deoxynivalenol on the mitochondrial membrane potentials and Bax, Bcl-2 expression of BHK-21 cells
摘要
Abstract
To investigate the effect of deoxynivalenol (DON)on the mitochondrial membrane potentials MMP) and Bax, Bcl-2 expression of hamster kidney cells (BHK-21 cell )in vitro, the change of early apoptosis, mitochondrial membrane potential and apoptosis-related proteins with Bax and Bcl-2 expression were measured by flow cytometry and immunocytochemical staining assay. The results showed that different concentration of DON could induce apoptosis for BHK-21 cells within the experimental concentration and cause apoptosis significantly as compared with control group (P ＜ O. 05 ). A marked decrease of mitochondrial membrane potential in a dose and time dependent manner were observed clearly by mitochondrial membrane potentials assay. Immunohistochemical staining analysis showed that, after 24 h treatment with DON, the expression of Bax was significantly higher, while Bcl-2 decreased. These result suggested that DON could induce apoptesis and the MMP disruptionin of BHK-21 cells in vitro. The essential mechanisms of apoptosis might be related to inducing the change of hepatocarcinoma cell cycle, the expression of the Bax increase and Bcl-2 decrease in BHK-21 cells.关键词
脱氧雪腐镰刀菌烯醇/仓鼠肾细胞(BHK-21)/线粒体膜电位/细胞凋亡/Bax/Bcl-2Key words
deoxynivalenol (DON) /hamster kidney cells( BHK-21 ) /mitochondrial membrane potentials(MMP) /apoptosis /Bax /Bcl-2分类
医药卫生引用本文复制引用
丁举静,何成华,刘俐君,马彦娜,张海彬..脱氧雪腐镰刀菌烯醇对BHK-21细胞的线粒体膜电位及Bax和Bcl-2蛋白表达的影响[J].南京农业大学学报,2011,34(2):101-106,6.基金项目
国家863计划项目(2007AA102429) （2007AA102429）
国家自然科学基金项目(A200748,A200955) （A200748,A200955）
江苏省农业科技自主创新基金项目(CX(08)135) （CX(08）
