中国输血杂志2011，Vol.24Issue(2)：122-125,4.
利用新型α-半乳糖苷酶进行B→O血型改造
B to O blood group conversion by new type α-galactosidase
王玲燕 1高红伟 1金泗虎 1李素波 1虞立霞 1鲍国强 1宫锋1
作者信息
- 1. 军事医学科学院野战输血研究所,北京,100850
- 折叠
摘要
Abstract
Objective To study the B to O blood group conversion by α-galactosidase from procaryote. Methods The α-galactosidase gene was cloned from Bacteriodes fragilis by PCR and inserted into the procaryotic expression vector. We induced soluble α-galactosidase by IPTG and used the ultrasonic disruption supernatant of E. coli to digest the B group red blood cells. Results The activity of α-galactosidase was about 2 U/mi and the molecular weight of α-galactosidase was about 64.6 kD. B antigen was cleaned within 2 hours by 30 μl supematant at 26℃, pH 6.8 completely. The enzymatically converted red blood cells did not react with anti-B and anti-A monoclonal antibodies. Conclusion Group B red blood cells could be effectively converted to group O by recombinant procaryote α-galactosidase.关键词
α-半乳糖苷酶/血型改造/B抗原分类
临床医学引用本文复制引用
王玲燕,高红伟,金泗虎,李素波,虞立霞,鲍国强,宫锋..利用新型α-半乳糖苷酶进行B→O血型改造[J].中国输血杂志,2011,24(2):122-125,4.
