郑州大学学报（医学版）2011，Vol.46Issue(2)：259-263,5.
人抗黏液病毒A基因重组腺病毒体外抗乙型肝炎病毒的作用
Methylation and imprinting status of p73 gene in tissue of Non-Hodgkin's lymphoma
毛国强 1彭明利 2赵瑞秋 1黄琴 1刘爱平 1任红 2许红梅1
作者信息
- 1. 重庆医科大学儿童医院传染科,重庆,400014
- 2. 重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆医科大学病
- 折叠
摘要
Abstract
Aim: To study the relationship between the methylation and imprinting status of p73 gene promoter region and non-Hodgkin's lymphoma. Methods: A total of 40 lymph node biopsy specimens, including 20 specimens of nonHodgkin's lymphoma( 16 specimens of B-cell lymphoma,4 specimens of T-cell lymphoma) and 20 specimens of reactive hyperplasia of lymph node. We detected the gene imprinting and promoter region CpG Island methylation status of p73 gene in these tissues by reversed transcript PCR with restriction fragment length polymorphism(RT-PCR-RFLP) method and methylation-specific polymerase chain reaction(MS-PCR) method, respectively. Results:There were eight cases of heterozygous phenotype of p73 gene in 20 cases of non-Hodgkin' s lymphoma,in which the number of cases of p73 gene monoallelic phenotype was seven; There were six cases of heterozygous phenotype of p73 gene in 20 cases of reactive hyperplasia of lymph node,in which the number of cases of p73 gene monoallelic phenotype was five. p73 ailelotype between the two关键词
重组腺病毒/人抗黏液病毒A基因/乙型病毒性肝炎病毒Key words
p73 gene/ meythlation/ imprinting status/ non-Hodgkin's lymphoma分类
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毛国强,彭明利,赵瑞秋,黄琴,刘爱平,任红,许红梅..人抗黏液病毒A基因重组腺病毒体外抗乙型肝炎病毒的作用[J].郑州大学学报（医学版）,2011,46(2):259-263,5.
