郑州大学学报（医学版）2011，Vol.46Issue(2)：263-266,4.
非霍奇金淋巴瘤组织中p73基因印迹状态及甲基化
Methylation and imprinting status of p73 gene in tissue of Non-Hodgkin' s lymphoma
崔美英 1张静静 1王冠男 1高献争 1张丹丹 1高冬玲 1李文才1
作者信息
- 1. 郑州大学第一附属医院病理科,郑州大学基础医学院病理学教研室,河南省肿瘤病理重点实验室,郑州,450052
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摘要
Abstract
Aim: To study the relationship between the methylation and imprinting status of p73 gene promoter region and non-Hodgkin's lymphoma. Mothods: A total of 40 lymph node biopsy specimens, including 20 specimens of nonHodgkin's lymphoma( 16 specimens of B-cell lymphoma,4 specimens of T-cell lymphoma) and 20 specimens of reactive hyperplasia of lymph node. We detected the gene imprinting and promoter region CpG Island methylation status of p73 gene in these tissues by reversed transcript PCR with restriction fragment length polymorphism(RT-PCR-RFLP) method and methylation-specific polymerase chain reaction(MS-PCR) method, respectively. Results:There were eight cases of heterozygous phenotype of p73 gene in 20 cases of non-Hodgkin' s lymphoma,in which the number of cases of p73 gene monoallelic phenotype was seven; There were six cases of heterozygous phenotype of p73 gene in 20 cases of reactive hyperplasia of lymph node,in which the number of cases of p73 gene monoallelic phenotype was five. p73 allelotype between the two groups had no statistical significance ( P ＞ 0. 999 ). The methylation rate of p73 gene in non-Hodgkin' s lymphoma was 35.0％, while the rate of control group was 0 ( P = 0.008 ). Conclusion: Abnormal methylation of p73 gene may be related to non-Hodgkin's lymphoma.关键词
p73基因/甲基化/印迹状态/非霍奇金淋巴瘤Key words
p73 gene/ meythlation/ imprinting status/ non-Hodgkin's lymphoma分类
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崔美英,张静静,王冠男,高献争,张丹丹,高冬玲,李文才..非霍奇金淋巴瘤组织中p73基因印迹状态及甲基化[J].郑州大学学报（医学版）,2011,46(2):263-266,4.
