临床输血与检验2011，Vol.13Issue(2)：104-107,4.DOI:10.3969/J.issn.1671-2587.2011.02.003
戊型肝炎病毒不同生物标志物的关联和基因分型
Analysis of Relationship among Hepatitis E Virus Biomarkers and Viral Genotypes
摘要
Abstract
Objective To analyze the relationship among hepatitis E virus (HEV) biomarkers and their genotype distribution and epidemiology, to provide the early diagnosis of HEV infection. Methods 46 sera were collected from the patients with clinical diagnosis of sporadic acute hepatitis in Anqing municipal hospital. All the sera were tested for antiHEV IgG, IgM antibodies and RNA with real-time fluorescent RT-PCR, respectively. To apply reverse transcriptase PCR and type-specific primers on partial cases of HEV RNA specimens for sequencing and genotyping. Results Among 46 clinical diagnostic hepatitis E cases, 36 appeared with rising HEV-IgG title, 41 and 39 were positive respectively with HEVIgM and HEV RNA. The proportion of accordance between HEV-IgM and HEV-IgG, HEV-IgM and HEV RNA, HEVIgG and HEV RNA were separately 88.2％, 85. 3％ and 83. 8％. A 150-nt nucleotide fragment within HEV RNA ORF2 was amplified in 15 samples. All viral strains belonged to genotype Ⅳ. Conclusion The combinational detection of immunological with viral biomarkers would be helpful in early diagnosis of HEV infection. The dominant genotypes of clinical hepatitis E patients in Anqing district belonged to genotype 4.关键词
戊型肝炎病毒/RT-PCR/基因型Key words
Hepatitis E virus/ RT-PCR/ Genotype分类
医药卫生引用本文复制引用
刘海燕,胡安群,郑英杰,陆一涵,夏玉刚,黄升海..戊型肝炎病毒不同生物标志物的关联和基因分型[J].临床输血与检验,2011,13(2):104-107,4.基金项目
本课题受安徽高校省级自然科学研究计划项目(No.KJ2008B44ZC)资助 （No.KJ2008B44ZC）
