四川大学学报（自然科学版）2011，Vol.48Issue(2)：441-444,4.DOI:10.3969/j.issn.0490-6756.2011.02.036
Sanglifehrin A产生菌Streptomyces flaveolus DSM 9954遗传转化系统的建立
The genetic system development of Sanglifehrin A producing host Streptomyces flaveolus DSM 9954
摘要
Abstract
Sanglifehrin A is produced by Streptomyces flaveolus DSM 9954.TSB culture was selected as its growing culture, ISP-4 as sporing culture.Antibiotic resisting test showed S.flaveolus DSM 9954 was sensitive to all the chosen antibiotics.The genetic transformation system of S.flaveolus DSM 9954 was successfully developed either by the protoplast transformation or conjugation between S.flaveolus and E.coli.And the plasmid pKC1139 was rescued from the transformants and confirmed by EcoR Ⅰ digestion, which proved the success of conjugation between S.flaveolus and E.coli.These results laid the foundation for studying Sanglifehrin A's biosynthesis pathway and producing its analogues.关键词
Sanglifehrin A/原生质体转化/接合转移Key words
Sanglifehrin A/ protoplast transformation/ conjugation分类
生物科学引用本文复制引用
刘运,陶科,史冠莹,瞿旭东,侯太平..Sanglifehrin A产生菌Streptomyces flaveolus DSM 9954遗传转化系统的建立[J].四川大学学报（自然科学版）,2011,48(2):441-444,4.基金项目
国家自然科学基金(30800034) （30800034）
