河南农业科学2011，Vol.40Issue(2)：142-145,4.
鸡新城疫病毒新乡株ND-XX10的分离与鉴定
Isolation and Identification of Chicken Newcastle Disease Virus Xinxiang Strain
摘要
Abstract
To isolate field Newcastle disease virus (NDV) stains,10-day-old SPF chicken embryos was inoculated with suspected sample collected from disease chicken. The virus genomic RNA is extracted and the main region of F gene (1-631 bp) fragment was amplified by RT- PCR and identified by DNA sequencing. The results showed that a new isolate, namely ND- XX10, was obtained. The deduced amino acid sequence of the F protein of ND- XX10 isolate at the cleavage site was 112 R - R - Q - K - R - F117, which is typically found in virulent NDV isolates. The amino acid residues at position 101 and 121 are K and V,respectively. Phylogenetic analysis showed that the isolate belonged to genotype Ⅶ e.关键词
新城疫病毒/F基因/RT-PCRKey words
NDV/ F gene/ PT - PCR分类
农业科技引用本文复制引用
银梅,赵坤,陈桂香,宁红梅,贾文科,王选年..鸡新城疫病毒新乡株ND-XX10的分离与鉴定[J].河南农业科学,2011,40(2):142-145,4.基金项目
国家"十一五"科技支撑计划重大项目子课题(2006BAK02A21/2) （2006BAK02A21/2）
河南科技学院骨干教师资助项目(200604) （200604）
