生物加工过程2011，Vol.9Issue(2)：49-52,4.DOI:10.3969/j.issn.1672-3678.2011.02.010
卷枝毛霉孢子原生质体的制备与再生
Preparation and regeneration of protoplasts of Mucor circinelloides spores
陈清霞 1江贤章 1刘锦清 1宋芬芬 1黄建忠1
作者信息
- 1. 福建师范大学,生命科学学院,工业微生物教育部工程研究中心,福建省现代发酵技术工程研究中心,福州,350108
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摘要
Abstract
To construct a stable genetic transformation system, the protoplasts of Mucor circinelloides sp.EIM- 10 spores were prepared by using enzymatic methods. The influences of enzyme system, osmotic pressure stabilizer, enzymolysis time, and temperature on the preparation and regeneration of the protoplasts of Mucor circinelloides sp. EIM-10 spores were studied. The optimum preparation conditions were established by using enzyme system composed of 1％ cellulase and 2％ lywallzyme with 0. 5 mol/L NaCl being used as osmotic pressure stabilizer at 32 ℃ for 2. 5 h. When regenerated,0. 5 mol/L NaCl was chosen as osmotic pressure stabilizer by using the double-decked culture medium. Under the conditions, the formation of protoplast was 1.2 × 106/mL and the regeneration rate was 70.5％.关键词
γ-亚麻酸/卷枝毛霉/原生质体/孢子分类
生物科学引用本文复制引用
陈清霞,江贤章,刘锦清,宋芬芬,黄建忠..卷枝毛霉孢子原生质体的制备与再生[J].生物加工过程,2011,9(2):49-52,4.
