生物加工过程2011，Vol.9Issue(2)：58-63,6.DOI:10.3969/j.issn.1672-3678.2011.02.012
Ⅱ型胶原-硫酸软骨素支架的制备及组织工程软骨的构建
Preparation of type Ⅱ collagen-CS scaffolds and application in tissue engineering cartilage
摘要
Abstract
The preparation and characteration of type Ⅱ collagen-chondroitin sulfate ( CS ) scaffolds and the application in tissue engineering cartilage were studied. Porous type Ⅱ collagen-CS scaffolds were prepared using variable concentration of 1-ethyl-3 ( 3-dimethyl aminopropyl) carbodiimide ( EDC ). The physico-chemical properties of the collagen scaffolds were evaluated. Then,isolated chondrocytes from the rabbit articular cartilage were cultured in porous type Ⅱ collagen scaffolds in the presence of covalently attached CS up to 14 d. Cell proliferation, the total amount of proteoglycans and type Ⅱ collagen retained in the scaffold and chondrocytes morphology were evaluated. Results suggested that EDC-crosslinking was proved to be mechanically stable for scaffolds with increasing EDC concentration. On concentration of EDC higher than 7 mg/mL, the crosslinking stability was obtained. Cell proliferation and the total amount of proteoglycans and type Ⅱ collagen retained in the scaffolds were higher in type Ⅱ collagen-CS scaffolds. Scanning electron microscopy revealed a chondrocyte distributed throughout the porous surface of scaffolds with ellipse.关键词
Ⅱ型胶原-硫酸软骨素支架/EDC交联/软骨细胞/组织工程分类
医药卫生引用本文复制引用
曹慧,许时婴..Ⅱ型胶原-硫酸软骨素支架的制备及组织工程软骨的构建[J].生物加工过程,2011,9(2):58-63,6.基金项目
上海市自然科学基金资助项目(11ZR1424400) （11ZR1424400）
上海高校选拔培养优秀青年教师科研专项基金资助项目(Slg08030) （Slg08030）
