生物加工过程2011，Vol.9Issue(2)：64-67,4.DOI:10.3969/j.issn.1672-3678.2011.02.013
分子印迹技术应用于血清中地高辛的快速检测
Rapid detection of digoxin in serum samples by molecular imprinted biosensor
摘要
Abstract
The biosensor was prepared by molecular imprinted technique with digoxin-specific adsorption properties. Firstly the imprinted polymer particles were prepared. Then, particles and the agarose were mixed in proportion. The molecular imprinted polymer film sensor for the digoxin was fixed by the mixture on a glassy carbon electrode ( GC ). The sensor had specific combination of template molecule-digoxin and its electrochemical signals associated with the template concentration. Thus,it could be used to detect digoxin levels in serum. The molecular imprinted biosensor had advantages of simple, low cost, rapid detection, high specificity, and good stability. The detection limit of the biosensor was 1.28 nmol/L and detection time was 5 min.关键词
分子印迹传感器/地高辛/聚合物/血清/快速检测分类
化学化工引用本文复制引用
李敏,张娟琨,张子怡,张彬彬,范婷,吴萍..分子印迹技术应用于血清中地高辛的快速检测[J].生物加工过程,2011,9(2):64-67,4.基金项目
科技部资助项目(2009GJA10047) （2009GJA10047）
天津市科委资助项目(08ZXCXSH053,09ZCZDSF04200) （08ZXCXSH053,09ZCZDSF04200）
