中国兽医科学2011，Vol.41Issue(3)：229-234,6.
甘肃犬瘟热病毒流行株N蛋白和H蛋白基因的序列分析
Sequence analysis of nucleoprotein and hemagglutinin genes of wild type canine distemper virus isolated from dogs in Gansu Province
摘要
Abstract
The nucleoprotein(N) gene and hemagglutinin(H) gene were amplified from 3 strains of canine distemper virus(CDV) isolated from dogs in Gansu Province by RT-PCR. Subsequently, the nucleotides of these two genes were sequenced and analyzed by Clustal V of DNAstar. The N gene shared from 92.5 ％ to 99.9 ％ homology with those of the CDV strains available in GenBank. The H gene shared from 81.7％ to 100％ homology with those of the CDV strains from other regions. The phylogenetic tree based on the amino acid sequences deduced from the nucleotides sequences of the N gene indicated that the strains of CDV had remarkable difference and characteristics among different regions, but the phylogenetic tree based on the deduced H protein's amino acid sequences showed that the CDV strains were not different among the different regions. The above results showed that the 3 CDV strains from Gansu were classified as wild type. The mutations in H gene were more than that in N gene and N gene were able to display the relationship between CDV strain and region.关键词
犬瘟热病毒/N基因/H基因/序列分析分类
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王小辉,王旭荣,郭庆勇,李建喜,李世宏..甘肃犬瘟热病毒流行株N蛋白和H蛋白基因的序列分析[J].中国兽医科学,2011,41(3):229-234,6.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(BRF080302) （BRF080302）
