中国兽医科学2011，Vol.41Issue(3)：240-244,5.
鸡马立克氏病病毒HC135分离株的致病性
Pathogenicity of Marek's disease virus field strain HC135
摘要
Abstract
Peripheral blood lymphocytes of the diseased chickens from commercial chicken flocks experienced significant Marek's disease visceral tumour in Liaoning Province were collected,and were inoculated into monolayer of duck embryo fibroblast. The isolated Marek's disease virus(MDV) was obtained by plaque clone, and named strain HC135. To examine the pathogenicity of this isolate, unvaccinated SPF chickens were challenged with strain HC135. Morbidity and mortality were 100％ (10/10) and 30％ (3/10),respectively. HVT vaccine alone or combined with SB1 vaccine strains provided poor protection when the SPF chickens were challenged with this strain. Protection index was 22.2 and 40.0, respectively. Vaccination to chickens with HVT vaccine alone or combined with SB1 vaccine strains did not protect satisfactorily against the strain HC135. It had the characteristics of vv+ MDV strains, indicating the occurrence of vv+ MDV in Chinese commercial flocks. Meq gene nucleotide were determined and compared with 14 reference strains. The results showed that there was no nucleotide deletion or insertion in the ORF of the Meq gene of the isolate. But there were nucleotide mutations,and the nucleotide changes would lead to changes in the predicted amino acid sequences of the isolate. Their variations are corresponded to the high virulence MDV strains characteristic.关键词
马立克氏病病毒/致病性/Meq基因/分离毒株分类
农业科技引用本文复制引用
张艳萍,刘长军..鸡马立克氏病病毒HC135分离株的致病性[J].中国兽医科学,2011,41(3):240-244,5.基金项目
国家重点实验室基本科研业务费专项(SKLVBP201008) （SKLVBP201008）
中央级公益性科研院所基本科研业务费专项(1610302011002) （1610302011002）
