中国兽医科学2011，Vol.41Issue(3)：245-249,5.
犬源韦氏巴贝斯虫的分子鉴定和系统发育研究
Molecular identification and phylogenetic analysis of Babesia vogeli from dogs
摘要
Abstract
The 18 S rRNA gene of Babesia was amplified by PCR using a pair of conserved primers(5-22F and 1661R) from canine whole blood genomic DNA extracted from suspected pet dogs in Guangzhou and Foshan. The recombinant plasmids were identified by PCR and the positive clones were sequenced. A 1 677 bp sequence in length was obtained that was identical to the 18 S rRNA gene of Babesia vogeli published previously(AB083374),meanwhile,compared with the 18 S rRNA gene of B. canis(AY072926),B.rossi (L19079) and B. gibsoni (AF271081) available in GenBank, the sequences similarities were 98.0 ％,95.2％ and 93.4％ ,respectively. We confirmed the prevalence of B. vogeli in pet dogs in China by molecular approach for the first time,and the classification of Babesia was discussed in the present study.关键词
犬/韦氏巴贝斯虫/18/S rRNA基因/系统进化分析分类
农业科技引用本文复制引用
李高强,张浩吉,张更利,蒲文珺,周庆国,丁巧玲,李金平,李国清..犬源韦氏巴贝斯虫的分子鉴定和系统发育研究[J].中国兽医科学,2011,41(3):245-249,5.基金项目
广东省自然科学基金项目(10152800001000007) （10152800001000007）
