生物骨科材料与临床研究2011，Vol.8Issue(2)：3-7,5.DOI:10.3969/j.issn.1672-5972.2011.04.002
Ⅰ型胶原-壳聚糖复合材料的生物相容性研究
Biocompatibility researches of type Ⅰ collagen -chitosan scaffold
摘要
Abstract
Objective To evaluate the biocompatibility of the type Ⅰ collagen - chitosan scaffold for tissue engineering.Methods Cultured bone marrow mesenchymal stem cells (BMSCs) in 4 different groups(A,B,C and D).Used tetrazolium salt (MTT) colorimetric to assay the growth of different groups,and measure the optical density (OD)by microplate reader,then calculate the relative growth rate (RGR％).Mixed the scafford extracts with fresh blood of the rabbit,the grade of hemolysis was made in the measures of optical density at 492nm. Results BMSCs of group A, group B and group C were growing well, and proliferating gradually in the 1st,3rd and 5th day. The positive control group (D) cells rounded and wrinkled in the 1st day, then, cells were shrinkage death. The optical density were no significant statistical differences between group A,B and C (P ＞0.05), but all had significant statistical differences with group D (P ＜0.05).Hemolysis percentage of the scafford was 4.1％. Conclusion Ⅰ collagen - chitosan scafford has no significant cytotoxicity, and accords with the biomaterial standards of tissue engineering.关键词
胶原-壳聚糖材料/细胞毒性试验/溶血试验分类
医药卫生引用本文复制引用
张永强,任志鹏,杨自权,孙晓丹,卫小春..Ⅰ型胶原-壳聚糖复合材料的生物相容性研究[J].生物骨科材料与临床研究,2011,8(2):3-7,5.基金项目
国家自然基金项目(30973048) （30973048）
科技部国际合作重点项目(2008GR0403) （2008GR0403）
国家人力资源与社会保障部2009年度留学回国人员科技活动择优资助项目 （）
