上海海洋大学学报2011，Vol.20Issue(2)：161-166,6.
中华鳖黄河群体选育世代F1、F2及F3遗传变异微卫星分析
Microsatellite analysis of genetic variation in F1, F2 and F3 selected generations of Yellow River populations of Chinese soft-shelled turtle,Trionyx sinensis
摘要
Abstract
To assess genetic variations during genetic selection of Chinese soft-shelled turtle (Trionyx sinensis), the specimens of Chinese soft-shelled turtle , collected from three selected generations ( F1 , F2 and F3 ) of Yellow River population as testing group , and their base population (FO ) as well as the Taihu population as control group, were amplified by ten pairs of microsatellite primers.The result showed that:( 1 ) A total of 240 alleles were detected in all examined loci.In all five populations the value of average allele number ( Na ) were 4.60 - 5.30, the value of average of effective number of alleles ( Ne ) were 3.24 - 3.59;the value of average observed heterozygosity ( H0 ) were O.483 3 - O.530 O, the value of average expected heterozygosity (He) were O.594 9 -0.645 9 and the value of average polymorphism information content (CPI) were O.534 0 -0.568 4.(2)The three selected generation' s heredity multiple parameter Ho, He and CPI demonstrated decreased identically, along with the increases of selected generations.It indicated that the mass selection targeted on phenotypes such as growth, body color, body shape, produced a detectable effect on the genetype.(3)The phylogenetic tree showed that studied five populations were clustered into two groups, the first group consisted of the four Yellow River populations while the second group belongs to Taihu Lake population.It indicated that a significant genetic differentiation between Yellow River populations and Taihu Lake population existed and has not been changed by current three generations selection.关键词
中华鳖/黄河/选育/微卫星/遗传变异分类
农业科技引用本文复制引用
张志允,李思发,蔡完其,谢中富,殷黎明..中华鳖黄河群体选育世代F1、F2及F3遗传变异微卫星分析[J].上海海洋大学学报,2011,20(2):161-166,6.基金项目
浙江省绍兴市重点农业科研项目(2009A22027) （2009A22027）
