生殖医学杂志2011，Vol.20Issue(2)：141-145,5.DOI:10.3969/j.issn.1004-3845.2011.02.017
二至天癸颗粒对克罗米芬诱导排卵小鼠子宫内膜整合素β3表达的影响
Effect of Erzhi Tiangui Granule on the expression of endometrial beta3-integrin in the mice of induced ovulation with clomifene citrate
摘要
Abstract
Objective: To explore the effect of Erzhi Tiangui Granules (ETG) on the expression of endometrial beta3-integrin in mice of induced ovulation with clomifene citrate (CC).Methods: Forty Kunming female mice with ovulation failure were randomized into TCM group (treated with CC and ETG) and CC group (treated with CC only), while other 20 healthy mice of the same age were as normal controls.Their implantation rate on the fourth day of pregnancy (PD4) and the morphological changes of endometrium were observed, and the expression of endometrial beta3-integrin was detected by immunohistochemistry.Results: More mice had their oestrous cycle recovered in TCM group than in CC group (P＜0.05).The endometrial morphological changes on PD4 in TCM group were significantly more suitable for implantation(P＜0.05).The immunostaining scores of beta3-integrin in luminal epithelium, glandular epithelium and stroma of endometrium were all higher in TCM group than in CC group (P＜0.05).The number of implanted blastocysts, implantation rate, and the expression of beta3-integrin in glandular epithelium and stroma in TCM group were comparable to those in normal controls (P＞0.05), while expression of beta3integrin in luminal epithelium was lower in TCM group than in normal controls (P＜0.05).The expressions of beta3-integrin in luminal and glandular epithelium were both higher than that in stroma in all three groups (P＜0.05).Conclusions: ETG can help to restore the oestrous cycle and improve the expression of endometrial beta3-integrin and implantation rate in the mouse with ovulation failure.关键词
二至天癸颗粒/克罗米芬/整合素β3引用本文复制引用
连方,李婷婷,孙振高..二至天癸颗粒对克罗米芬诱导排卵小鼠子宫内膜整合素β3表达的影响[J].生殖医学杂志,2011,20(2):141-145,5.基金项目
国家自然科学基金资助项目(No.30672706) （No.30672706）
