摘要
Abstract
Objective To investigate the effect of synthetic DNA sequences containing unmethylated cytosine-guanine motifs on Lewis lung cancer treated with carbplatin in mice.Methods The tumor-bearing mouse model was induced by injecting Lewis lung cancer cells into the right infra-axillary of 32 C57BL mice; the animals were evenly randomized into 4 groups: control group without treatment,carboplatin group treated with carboplatin only (0.32 mg/day,on day 1,2,3,4,5) ,CpG group receiving CpG ODN1826 0.05 mg on day 1,2,3,4,5; CpG + carboplatin group treated with CpG ODN1826 6 hours before carboplatin.The non tumor-bearing C57BL mice were used as blank controls.Tumor growth and tumor weight were observed in all groups.Serum TNF-α and 1L-12 levels,the spleen index and thymus index were measured.Results The Lewis lung cancer-bearing model was successfully established in mice.The tumor volumes of treated groups were smaller than those in control group ( P ＜ 0.01 ), and the tumor volumes of CpG + carboplatin group was the smallest.The tumor inhibitory rates were 23.4％ in carboplatin group,21.5％ in CpG group C and 48.4％in CpG + carboplatin group.The serum concentrations of TNF-α and IL-12 were (105.8 ±9.9) μg/L and (16.56 ±0.99) ng/L in blank group, ( 125.5 ± 18.4) μg/L and ( 17.87 ± 1.11 ) ng/L in control group, ( 199.0 ± 8.4) μg/L and ( 24.56 ± 1.2 ) ng/L in CpG group, (70.4 ± 8.7) μg/L and ( 12.50 ± 1.15 ) ng/L in carboplatin group, ( 127.8 ± 17.3 ) μg/L and ( 16.32 ± 0.73 ) ng/L in CpG plus carboplatin group.The spleen and thymus index was ( 8.64 ± 0.82 ) mg/g and ( 2.55 ± 0.24 ) mg/g respectively in blank group;( 11.11 ± 1.6) mg/g and (2.96 ±0.29) mg/g in control group,( 16.01 ± 1.83) mg/g and (3.31 ±0.27) mg/g in CpG group, (8.27± 1.12) mg/g and ( 1.81 ± 0.8 ) mg/g in carboplatin group, ( 12.58 ± 1.58 ) mg/g and (2.46 ± 0.25 ) mg/8 in CpG with carboplatin group.All immune indexes in CpG group were higher than those in control group( P ＜0.05) ,and were higher than those in carboplatin group also; But,All immune indexes in carboplatin group were lower than those in control group(P ＜0.01 ).Moreover, all immune indexes in combination group were higher than those in carboplatin group( P ＜0.001 ).Conclusions CpG ODN1826 can dramatically increase the chemo-sensitivity of tumor cells by activating the immune system and elevate the immune function of tumor-bearing mice.关键词
肺肿瘤/药物疗法/肺肿瘤/病理学/寡脱氧核糖核苷酸类/治疗应用/卡铂/治疗应用/肿瘤坏死因子α/血液/动物实验分类
医药卫生
