环境工程学报2011，Vol.5Issue(4)：935-938,4.
河流沉积物中微生物DNA的提取方法比较研究
Comparative study of DNA extraction methods of microorganisms in river sediment
摘要
Abstract
DNA extraction of river sediment sample is one of the difficulties in the research of the river sediment microbial diversity. An extraction method of DNA, i.e. phenol with SDS measure was developed in this study, and was compared with the other two DNA extraction kits of OMEGA and BioDee. The above mentioned methods were evaluated by the results of DNA purity, PCR amplification and 16S rDNA clone library. The results showed that the OMEGA DNA extraction kit method was a simple way with high DNA purity. The phenol +SDS method obtained plenty of DNA, however a longer time was needed and with lower purity DNA. The extraction rate of BioDee extraction kit method was minimum,but no effect on the PCR results. However, the PCR production was not suitable to the next steps of the analysis of sediment microbial diversity. The above results showed that the DNA, which was obtained using OMEGA DNA extraction kit method and the phenol + SDS method, could be used for the next steps of PCR, 16S rDNA clone library, DGGE and other molecular tests.关键词
河流沉积物/微生物/DNA/提取方法Key words
river sediment/ microorganism/ DNA/ extraction method分类
资源环境引用本文复制引用
朱灵峰,张乐,王海燕,周岳溪,张召耀,王业耀,雷书凤..河流沉积物中微生物DNA的提取方法比较研究[J].环境工程学报,2011,5(4):935-938,4.基金项目
国家水体污染控制与治理科技重大专项(2008ZX07207-010) （2008ZX07207-010）
