国际神经病学神经外科学杂志2011，Vol.38Issue(2)：119-123,5.
人脑脊液对鼠神经干细胞增殖分化的影响
Effect of human cerebrospinal fluid on the proliferation and differentiation of rat neural stem cells
刘国平 1赵京涛 1方加胜2
作者信息
- 1. 湖南省湘潭市中心医院神经外科,湖南湘潭,411100
- 2. 中南大学湘雅医院神经外科,湖南长沙,410008
- 折叠
摘要
Abstract
Objective To investigate the effect of the injured brain cerebrospinal fluid(CSF) from traumatic brain injury and the hydrocephalus patients on the survival, proliferation and differentiation of neural stem cells (NSCs). Methods NSCs were isolated and cultured from brain of SD rat. The NSCs were cultured in human bloody CSF from the injured brain patients ( the experimental group) or CSF from the hydrocephalus patients ( the control group). The growth, proliferation and differentiation pattern of NSCs in two groups were observed at different times. The differentiated cells were identified by immunocytochemistry and their proportions were compared.Results The NSCs could survive and differentiate both in bloody CSF and hydrocephalus CSF, and part of the cells could grow up by floating in CSF more than ten days. On the second day of the cultivate process, the percentage of NSE positive cells (neurons) in experimental group is significantly higher than that of control group (P ＜ O.05 ). On the tenth day, the percentage of GFAP positive cells (astrocytes) in the experimental group is significantly higher than that of control group, while the percentage of NSE positive cells in the experimental group is significantly lower than that of control group ( P ＜ O.05 ). Conclusions The NSCs can survive, proliferate and differentiate both in the bloody CSF and hydrocephalus CSF of injured brain. There was different effect on the differentiation of NSCs between the two groups.关键词
脑脊液/神经干细胞/分化Key words
Cerebrospinal fluid ( CSF)/Neural stem cells ( NSCs)/Differentiation引用本文复制引用
刘国平,赵京涛,方加胜..人脑脊液对鼠神经干细胞增殖分化的影响[J].国际神经病学神经外科学杂志,2011,38(2):119-123,5.
