郑州大学学报（医学版）2011，Vol.46Issue(2)：179-182,4.
蛋白4.1R基因敲除小鼠的引种及繁殖
Introducing and breeding of protein 4.1 R gene knock out mice
摘要
Abstract
Aim: To introduce and reproduce 4. 1 R gene knock out mouse for exploring the functions of protien 4.1R.Methods:A total of 3 pairs of 4.1R gene knock out mice introduced from the USA were isolated and inspected according to the Animal Inspection and Quarantine Law of PR China. The introduced 4.1R knock out mice were breeding in IVC equipment according to the SPF Laboratory Animal Standard Operation Procedure. A pair of one male and one female homozygote were used to reproduce 4. 1R knock out mice and C57BL/6J mice were used as control. The body weights and the rate of femal reproduction, birth and survival between gene konck out and wild type mice were recorded regularly for further analysis. The genomic DNA from the murine tail tissue was subjected to PCR for genotyping. Restults:The inspection and quarantine results showed that the introduced 4.1R knock out mice were qualified for the SPF laboratory mouse grade of China.PCR results showed that 23 reproduced mice were 4. 1R gene knock out homozygotes. The average litter size was 5.8 and the survival rate was 74％. Though no significant difference of weight was found between wild type and 4. 1R knock out mice(Fgroups =4.035,P=0. 056;Flime =543.723,P ＜0.001 ;Fsnter =4.358,P =0.004). Conclusion: 4. 1R gene knock out mice has been introduced and reproduced successfully in China.关键词
基因敲除小鼠/蛋白4.1RKey words
gene knock out mouse/ protein 4. 1 R分类
生物科学引用本文复制引用
周晴晴,康巧珍,闫红霞,孙蕾,刘喜龙,李黎,汲翔,高艳锋,汲振余,祁元明..蛋白4.1R基因敲除小鼠的引种及繁殖[J].郑州大学学报（医学版）,2011,46(2):179-182,4.基金项目
国家自然科学基金资助项目 30972707,30873002 （）
河南省科技创新杰出青年基金资助项目 104100510004 （）
