郑州大学学报（医学版）2011，Vol.46Issue(2)：188-192,5.
尼古丁对脂多糖诱导的大鼠小胶质细胞激活和白细胞介素6表达的影响
Effects of nicotine on LPS-induced microglia activation and cytokine IL-6 expression in rats
摘要
Abstract
Aim :To observe the effects of nicotine on LPS-induced primary rat cortical microglia activation and cytokine IL-6 expression and to explore the possible mechanism of nicotine in PD. Methods:Primary rat cortical glial cells were cultured and microglial cells were purified,with or without nicotine and/or LPS. The IL-6 secretion concentration of microglia at different time was detected by ELISA and the activation of microglia( Ibal positive cells) was detected by immunocytochemical staining. Results: LPS induced activation of microglia, activated microglia increased the expression of activation marker lbal. 10 μg/L LPS induced the amount of cytokine IL-6 secretion of microglia at 4,8 and 24 h respectively were significantly higher than those of the control group ( t = 14. I 15,23. 530, and 32.076,P ＜ 0.05 ). The secretion of IL-6 had no significant difference between the amount at 4 and 8 h in microglia, which reached a peak; but pretreatment of cells with nicotine significantly inhibited microglia activation ( F = 13. 418, P = 0. 006 ) and the LPS-induced IL-6 production ( F =92. 569 ,P ＜0. 001 ). Conclusion: Nicotine may has protective effect against LPS-induced inflammatory response.关键词
帕金森病/小胶质细胞/脂多糖/尼古丁/白细胞介素6Key words
Parkinson' s disease/ microglia/ lipopolysaccharide/ nicotine/ IL-6分类
医药卫生引用本文复制引用
李治华,赵青赞,张华,任秀花,周明付,臧卫东..尼古丁对脂多糖诱导的大鼠小胶质细胞激活和白细胞介素6表达的影响[J].郑州大学学报（医学版）,2011,46(2):188-192,5.基金项目
国家自然科学基金资助项目30671103 （）
