临床神经病学杂志2011，Vol.24Issue(2)：114-117,4.
糖尿病对脑缺血大鼠细胞间黏附分子-1表达及脑组织和血-脑屏障损害的影响
Effect of diabetes on expression of intercellular adhesion molecule-1 and injury of brain tissue and bloodbrain barrier in cerebral ischemia rats
沈亚雯 1赵燕燕 1王文安2
作者信息
- 1. 202150,上海交通大学医学院附属新华医院崇明分院神经内科
- 2. 上海交通大学医学院附属新华医院神经内科
- 折叠
摘要
Abstract
Objective To investigate the effect of diabetes on expression of intercellular adhesion molecule-1 (ICAM-1) and injury of brain tissue and blood-brain barrier (BBB) in cerebral ischemia rats. Methods One hundred and twenty male SD rats were randomly divided into diabetes mellitus group ( DM group), normal control group (NC group), cerebral ischemia with diabetes mellitus group (CD group) and cerebral ischemia group (CI group), and 30 rats in each group. The last two groups were further divided into 5 subgroups with cerebral ischemia 1 h, 3 h, 6 h, 12 h and 24 h, and 6 rats in each subgroup. Diabetic rat models were made by intraperitoneal injection of streptozotocin, and focal cerebral ischemia models were made by intravascular thread method. Expression level of ICAM-1 mRNA was measured by reverse transcription PCR. The infarction volume and the degree of BBB injury were detected by TTC staining and Evans blue injection respectively. Results The expression of ICAM-1 mRNA in each subgroup of CI group was significantly higher than in NC group ( all P ＜ 0. 05). And the expression of ICAM-1 mRNA in each subgroup of CD group was significantly higher than in CI group ( all P ＜ 0. 05 ). The infarction volume and the open degree of BBB of CD group were significantly bigger than that of CI group ( all P ＜ 0. 05 ). Conclusions Diabetes may up regulate the expression of ICAM-1 of cerebral ischemia rats, and can exacerbate the injury of brain tissue and BBB. It indicates that the up regulation of ICAM-1 expression may be one of the mechanisms of diabetes's aggravating cerebral ischemia injury.关键词
脑缺血/糖尿病/细胞间黏附分子-1/血-脑屏障Key words
cerebral ischemia/ diabetes mellitus/ intercellular adhesion molecule-1/ blood-brain barrier分类
医药卫生引用本文复制引用
沈亚雯,赵燕燕,王文安..糖尿病对脑缺血大鼠细胞间黏附分子-1表达及脑组织和血-脑屏障损害的影响[J].临床神经病学杂志,2011,24(2):114-117,4.
