临床神经病学杂志2011，Vol.24Issue(2)：118-120,3.
糖尿病大鼠脑组织紧密连接蛋白Occludin 表达的变化
Change of fight junction protein Occludin expression in brain tissue in diabetic rats
余爱勇 1邓星奇 1赵玉武 2王继芹 1刘梅1
作者信息
- 1. 201100,上海市闵行区中心医院急诊科
- 2. 上海交通大学附属第六人民医院神经内科
- 折叠
摘要
Abstract
Objective To observe the change of tight junction protein Occludin expression in brain tissue in diabetic rats. Methods Thirty adult male SD rats were randomly divided into 1 month diabetic group, 3 month diabetic group and normal control group. The diabetic rats were induced by streptozotocin intraperitoneal injection.The level of Oeeludin mRNA and protein in brain tissue of the rats was detected by reverse transcription PCR and Western blot. Results The levels of Occludin mRNA in brain tissue of 1 month diabetic group and 3 month diabetic group [ (0.20 ± 0.21 ) , (0.06 ± 0.02 ) ] were significantly lower than normal control group ( 1.00 ± 0.00) ( all P ＜ 0. 05). And the level of Occludin mRNA in 3 month diabetic group was significantly lower than that in 1 month diabetic group (P ＜ O. 05 ). The levels of Occludin protein in 1 month diabetic group and 3 month diabetic group [ (0.58 ± 0.01 ), (0.29 ± 0.01 ) ] were significantly lower than that in normal control group ( 1.02 ± 0.06) ( P ＜0. 05-0.01 ). And the level of Occludin protein in 3 month diabetic group was significantly lower than that in 1 month diabetic group (P ＜ 0. 05 ). Conclusions The Occludin expression in brain tissue in diabetic rats is decreased, and the decrease get more obvious with the progress of diabetes. It indicated that the blood-brain barrier is broken down by diabetes.关键词
糖尿病/紧密连接蛋白/Occludin/血-脑屏障Key words
diabetes/ tight junction protein/ Occludin/ blood-brain barrier分类
医药卫生引用本文复制引用
余爱勇,邓星奇,赵玉武,王继芹,刘梅..糖尿病大鼠脑组织紧密连接蛋白Occludin 表达的变化[J].临床神经病学杂志,2011,24(2):118-120,3.
