军事医学2011，Vol.35Issue(3)：219-222,4.
紫杉醇热敏脂质体制备及抗肿瘤作用评价
Preparation of thermosensitive liposomes containing paclitaxel and their anticancer effect
摘要
Abstract
Objective To prepare the thermosensitive liposomes containing paclitaxel (PrL)and study their anticancer effect. Methods Both PTL and paclitaxel liposomes (PL) were prepared by the thin-film method. The HPLC method was established to determine paclitaxel concentration in vivo and in vitro. The model of mice Lewis sarcoma was established and divided into four groups randomly :control,PTX,PL and PTL groups. Each group was administrated with drug and local hyperthermia for 30 min. The volume and weight of tumor were detected. Results The mean particle size of the liposomes ranged 70 - 140 nm and the drug entrapment efficiency was about 99％. The phase transition temperature of PTL was about 42℃. In vivo, the study of thermosensitive characteristics of PTL indicated that drug content in heated location was significantly higher than that of the unheated. The inhibition ratio of tumor in PTX,PL and PTL groups was 49.79％ ,57.40％ and 75.66％, respectively. Conclusion Compared with PTX and PTL, PTL has an evident thermosensitive characteristic and increases the anticancer effect of paclitaxel remarkably when combined with hyperthermia.关键词
紫杉醇/脂质体/热敏/抗肿瘤作用分类
医药卫生引用本文复制引用
尤晓惠,张慧,潘见,付盟,梅兴国,胡富强..紫杉醇热敏脂质体制备及抗肿瘤作用评价[J].军事医学,2011,35(3):219-222,4.基金项目
国家"973"计划资助项目(2009CB30305) （2009CB30305）
国家重大新药创制科技重大专项资助项目(2009ZX09301-002) （2009ZX09301-002）
